Used in cell signaling research to quantify intracellular inositol triphosphate (IP3) levels, this ELISA kit enables accurate measurement of IP3 concentration in biological samples such as cell lysates and tissue extracts. It is widely applied in studies involving G-protein coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), where activation leads to phospholipase C-mediated production of IP3. By measuring IP3 dynamics, researchers investigate calcium signaling pathways, cellular responses like secretion, proliferation, and apoptosis, and pathophysiological mechanisms in diseases including cancer, diabetes, and neurological disorders. The assay is suitable for high-throughput screening and drug development where modulation of signaling pathways is assessed.