Used in immunological research to quantify Interleukin-35 (IL-35) levels in biological samples such as serum, plasma, and cell culture supernatants. Enables researchers to study the role of IL-35 in immune regulation, inflammation, and autoimmune diseases like multiple sclerosis, rheumatoid arthritis, and type 1 diabetes. Commonly applied in studies involving regulatory T cells and B cells due to IL-35’s immunosuppressive properties. Supports investigation into cancer immunology by measuring IL-35 expression in tumor microenvironments and its impact on immune evasion. Suitable for preclinical studies evaluating therapeutic interventions targeting the IL-35 pathway. Provides a reliable, sensitive, and high-throughput method for cytokine detection in experimental models and clinical research settings.