Used in biomedical research to detect and quantify levels of human I-Factor (also known as Factor I) in plasma and serum samples. This kit is essential for studying complement system regulation, as I-Factor plays a critical role in controlling complement activation by cleaving C3b and C4b. Researchers use this assay in immunology, nephrology, and autoimmune disease studies, particularly in conditions like atypical hemolytic uremic syndrome (aHUS), age-related macular degeneration, and systemic lupus erythematosus (SLE), where complement dysregulation is involved. The ELISA format allows for high-throughput, sensitive, and specific measurement, making it suitable for both clinical and experimental settings.