Used in biomedical research to quantify levels of Collagen IV in biological samples such as serum, plasma, and tissue homogenates. Widely applied in studies related to basement membrane integrity, liver fibrosis, kidney disease, and cancer progression. Helps assess extracellular matrix remodeling and is valuable in evaluating chronic disease markers, particularly in models of diabetic nephropathy and hepatic fibrosis. Commonly used in preclinical research and drug development to monitor disease progression and therapeutic response.