Used in research to quantify FNDC5 protein levels in biological samples such as serum, plasma, and tissue extracts. Helps in studying the role of FNDC5 in metabolic regulation, exercise physiology, and its link to conditions like obesity, diabetes, and neurodegenerative diseases. Commonly applied in preclinical studies to evaluate changes in irisin, a hormone derived from FNDC5 cleavage, in response to physical activity or therapeutic interventions. Supports investigation into energy homeostasis, mitochondrial function, and potential biomarker development for metabolic and neurological disorders.