Used in biomedical research to quantify Rat Collagenase II levels in tissue samples and biological fluids. Helps assess extracellular matrix degradation in studies related to inflammation, wound healing, fibrosis, and arthritis. Commonly applied in preclinical studies using rat models to evaluate disease progression and the effectiveness of therapeutic agents targeting collagen breakdown. Provides a reliable, sensitive, and specific method for detecting enzyme concentration, supporting research in immunology, orthopedics, and regenerative medicine.