Used in immunological research to quantify interleukin-27 levels in biological samples such as serum, plasma, or cell culture supernatants. Enables researchers to study the role of IL-27 in immune regulation, inflammation, and autoimmune diseases. Commonly applied in studies related to infectious diseases, cancer immunology, and therapeutic development where monitoring cytokine expression is critical. Provides a sensitive and specific method for detecting IL-27, supporting data accuracy in experimental models involving rat immune responses.