Used in biomedical research to quantify rat type I collagen levels in tissue samples, cell cultures, or biological fluids. Helps assess extracellular matrix formation, bone development, wound healing, and fibrosis progression in experimental models. Commonly applied in studies of osteoporosis, liver fibrosis, kidney disease, and skin regeneration. Enables researchers to evaluate the impact of drugs or therapeutic interventions on collagen synthesis and turnover in rat-based systems.