Used in biomedical research to quantify levels of 5-lipoxygenase (5-LO) protein in human cell or tissue samples. This assay helps investigate inflammatory processes, as 5-LO is involved in the production of leukotrienes, which are key mediators in asthma, arthritis, and other inflammatory diseases. Researchers use this kit to evaluate the effects of potential anti-inflammatory drugs, study immune cell activation, and explore the role of 5-LO in cancer and neurodegenerative conditions. The ELISA format allows for sensitive and specific detection, making it suitable for high-throughput screening in clinical and preclinical studies.