Used in immunological research to quantify levels of major histocompatibility complex class I molecules in mouse samples. Enables monitoring of immune activation, antigen presentation, and T-cell responses in experimental models. Commonly applied in studies of infection, autoimmunity, cancer immunotherapy, and transplant rejection. Provides a reliable, high-throughput method for detecting H-2 class I protein expression in cell lysates or tissue supernatants. Supports evaluation of vaccine efficacy and immune modulation in preclinical research.