Used in research settings to quantify melatonin levels in mouse biological samples such as serum, plasma, or tissue homogenates. This ELISA kit enables scientists to study circadian rhythm regulation, sleep disorders, reproductive cycles, and seasonal breeding patterns in mice. It is also applied in pharmacological studies assessing the impact of drugs or environmental factors on melatonin production. The assay provides high sensitivity and specificity, allowing accurate measurement of low-concentration melatonin, which is critical for longitudinal studies and experimental reproducibility in neuroscience, endocrinology, and chronobiology research.