Used in biomedical research to detect and quantify mesothelin protein levels in cell culture supernatants, serum, plasma, and other biological fluids. Widely applied in cancer research, particularly for studying mesothelioma, ovarian cancer, and pancreatic cancer, where mesothelin is overexpressed. Supports investigation into tumor biomarkers, therapeutic response monitoring, and development of targeted therapies such as CAR-T cells and antibody-drug conjugates. Provides a reliable, sensitive, and specific method for high-throughput screening in clinical and research laboratories.