Used in biomedical research to quantify human mast cell tryptase levels in biological samples such as serum, plasma, or tissue culture supernatants. This ELISA kit enables detection and measurement of mast cell activation, which is critical in studying allergic reactions, anaphylaxis, asthma, and mast cell disorders like mastocytosis. It is widely used in clinical research and drug development to evaluate immune responses and inflammatory processes involving mast cells. The assay provides high sensitivity and specificity, allowing researchers to assess disease progression or therapeutic efficacy in experimental models and patient samples.