Used in biomedical research to quantify levels of MDM2 protein in cell lysates or tissue samples. Enables study of the MDM2-p53 interaction, which plays a critical role in regulating cell cycle and apoptosis. Commonly applied in cancer research to assess MDM2 expression in tumor models, evaluate response to therapeutic agents, and investigate mechanisms of p53 pathway suppression. Provides a reliable, high-throughput method for monitoring MDM2 modulation in drug development and molecular biology studies.