Used in research settings to measure melatonin levels in biological samples from bovine sources. Enables accurate quantification of melatonin concentration in serum, plasma, or tissue extracts. Commonly applied in studies related to circadian rhythm regulation, reproductive physiology, and seasonal breeding patterns in cattle. Supports investigation into the effects of environmental factors such as light exposure, stress, or nutrition on melatonin production. Also utilized in veterinary research to assess pineal function and neuroendocrine responses. Provides a reliable, high-throughput method for detecting melatonin with high sensitivity and specificity.