Used in biochemical and medical research to detect and quantify malate dehydrogenase enzyme levels in rat samples. Helps assess mitochondrial function and cellular metabolism in studies related to metabolic disorders, oxidative stress, and tissue damage. Commonly applied in preclinical research involving liver, heart, and skeletal muscle to evaluate organ injury or disease progression. Provides a reliable, sensitive method for measuring MDH concentration in serum, plasma, or tissue homogenates through enzyme-linked immunosorbent assay techniques.