Used to measure interleukin-4 (IL-4) levels in biological samples such as serum, plasma, or cell culture supernatants from mice. It is commonly applied in immunology research to study T-helper 2 (Th2) immune responses, allergic reactions, and inflammation. The kit helps assess cytokine production in experiments involving immune cell activation, vaccine development, and autoimmune or infectious disease models. Its high sensitivity and specificity make it suitable for detecting low concentrations of IL-4, enabling accurate quantification in small sample volumes.