Used to detect and quantify levels of mouse interferon-alpha (IFN-α) in biological samples such as serum, plasma, and cell culture supernatants. Commonly applied in immunology and virology research to study immune responses, particularly in mouse models of viral infection, autoimmune diseases, and vaccine development. Enables researchers to assess the activation of innate immune pathways and evaluate the efficacy of therapeutic agents that modulate interferon production. The assay is sensitive, specific, and suitable for high-throughput analysis in preclinical studies.