Used to measure levels of interleukin-12 and interleukin-23 p40 subunit in biological samples such as serum, plasma, or cell culture supernatants from mice. This assay is widely applied in immunology research to study inflammatory responses, autoimmune diseases, and the role of Th1 and Th17 cell pathways. It helps evaluate the effectiveness of therapeutic agents targeting IL-12 or IL-23 signaling in models of diseases like multiple sclerosis, colitis, or psoriasis. The kit provides a sensitive and specific way to quantify cytokine production in experimental settings.