Used in research to measure Nesfatin-1 levels in biological samples such as serum, plasma, or tissue extracts. Helps in studying appetite regulation, energy balance, and metabolic disorders like obesity and diabetes. Commonly applied in neuroscience and endocrinology studies to understand the role of Nesfatin-1 in feeding behavior and stress responses. Also used in preclinical drug development to evaluate compounds affecting satiety pathways. Provides a reliable, sensitive method for quantifying Nesfatin-1 in mouse models, supporting translational research in metabolic and psychiatric conditions.