Used in research settings to detect and quantify nitroimidazole compounds in biological samples. Commonly applied in pharmacokinetic studies to monitor drug levels, especially for antibiotics and antiparasitic agents containing the nitroimidazole moiety. Helps assess metabolism and clearance in preclinical and clinical trials. Also utilized in environmental and food safety studies to detect residual nitroimidazole drugs in animal products, ensuring compliance with regulatory limits. The ELISA format offers high sensitivity and throughput, making it suitable for screening large sample numbers.