Used in biomedical research to quantify Nesfatin-1 levels in biological samples such as serum, plasma, and tissue homogenates. Enables investigation of Nesfatin-1's role in appetite regulation, energy homeostasis, and metabolic disorders like obesity and diabetes. Supports studies on the hormone’s involvement in stress responses, insulin secretion, and cardiovascular functions. Facilitates biomarker discovery and evaluation of therapeutic targets in neuroendocrine and metabolic disease research.