Used in biomedical research to measure the activation levels of NF-kB, a key transcription factor involved in inflammation, immune responses, and cell survival. The kit enables researchers to quantify NF-kB in nuclear extracts from cells or tissues, supporting studies in cancer, autoimmune diseases, and infectious diseases. It is widely applied in drug development to assess the efficacy of anti-inflammatory compounds and to investigate signaling pathways in cellular models. The ELISA format allows for high-throughput, sensitive, and specific detection, making it a valuable tool in both academic and pharmaceutical research settings.