Used in research settings to quantitatively measure Nesfatin-1 protein levels in biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit is particularly useful in studies related to appetite regulation, energy homeostasis, and metabolic disorders including obesity and diabetes. It enables researchers to investigate the role of Nesfatin-1 in neuroendocrine functions and its potential involvement in inflammatory and cardiovascular diseases. The assay provides high sensitivity and specificity, making it suitable for both clinical research and basic science investigations involving the Nesfatin-1 signaling pathway.