Used in biomedical research to quantify endothelial nitric oxide synthase (eNOS) levels in rat tissue samples, particularly in studies related to cardiovascular function and vascular health. Enables researchers to assess eNOS expression under various experimental conditions such as hypertension, atherosclerosis, or drug treatment. Commonly applied in preclinical studies to investigate endothelial dysfunction and the role of nitric oxide in regulating blood flow and vascular tone. Provides a reliable, sensitive, and specific method for measuring eNOS protein concentration in cell lysates and tissue homogenates.