Used in biomedical research to measure levels of nuclear transcription factor p65 (NF-κB p65) in cell or tissue samples. This kit enables researchers to quantify NF-κB activation, a key signaling molecule involved in inflammation, immune responses, cell survival, and stress. It is widely applied in studies related to inflammatory diseases, cancer, autoimmune disorders, and drug development where modulation of NF-κB pathways is a target. The ELISA format allows for sensitive and specific detection of p65 protein in nuclear extracts, supporting high-throughput analysis in preclinical research.