Used in biomedical research to quantify osteopontin levels in biological samples such as serum, plasma, and cell culture supernatants. Enables study of bone metabolism, inflammation, and tissue remodeling. Commonly applied in research on osteoporosis, arthritis, cancer metastasis, and cardiovascular diseases. Supports investigation of immune responses and cell signaling pathways involving osteopontin. Provides a reliable, sensitive, and specific measurement method in experimental models and clinical studies.