Used in biomedical research to measure levels of carboxylated osteocalcin in rat serum or plasma samples. This kit helps assess bone formation and mineralization processes, commonly applied in studies related to osteoporosis, metabolic bone diseases, and endocrine function. It enables researchers to evaluate the effects of drugs, hormones, or dietary interventions on bone metabolism in rat models. The assay is performed in a 96-well plate format, allowing high-throughput analysis with specific detection of the carboxylated form of osteocalcin, which reflects osteoblast activity and bone turnover status.