Used in biomedical research to detect and quantify pepsin levels in biological samples such as saliva, urine, or serum. Particularly valuable in studies related to gastroesophageal reflux disease (GERD), where pepsin presence in non-gastric tissues indicates reflux activity. Enables researchers to assess pepsin as a biomarker for acid reflux, laryngopharyngeal reflux (LPR), and related disorders. Commonly applied in clinical labs and pharmaceutical studies evaluating anti-reflux therapies or monitoring disease progression. The kit provides a sensitive and specific method for measuring mouse pepsin, supporting preclinical studies in animal models.