Used in biomedical research to quantify substance P levels in biological samples such as plasma, serum, cerebrospinal fluid, and tissue homogenates. Widely applied in studies related to neuroinflammation, pain pathways, and neurological disorders including migraine, depression, and Parkinson’s disease. Helps assess neuroimmune responses in experimental models of inflammation, stress, and injury. Also utilized in pharmacological studies to evaluate the efficacy of drugs targeting tachykinin receptors or neurokinin signaling. Provides a reliable, sensitive, and high-throughput method for measuring substance P in both preclinical and clinical research settings.