Used in biomedical research to detect and quantify pyridinoline crosslinks in biological samples, particularly in studies related to bone and connective tissue metabolism. The kit enables assessment of collagen degradation by measuring crosslink biomarkers in serum, urine, or tissue extracts. It is commonly applied in osteoporosis research, monitoring bone turnover, and evaluating the effectiveness of anti-resorptive therapies. The assay supports high-throughput analysis with high sensitivity and specificity, making it suitable for clinical and preclinical investigations into metabolic bone diseases and connective tissue disorders.