Used in biomedical research to quantify levels of human urokinase plasminogen activator (uPA) in cell culture supernatants, plasma, serum, and other biological fluids. The kit helps study the role of uPA in processes involving extracellular matrix degradation, such as cancer metastasis, inflammation, and tissue remodeling. It is widely applied in oncology research to assess uPA concentration as a biomarker for tumor aggressiveness and patient prognosis, particularly in breast, lung, and colorectal cancers. The assay supports high-throughput analysis and provides a reliable tool for evaluating therapeutic responses in experimental models targeting the plasminogen activation system.