Used in biomedical research to detect and quantify phosphorylated mTOR protein levels in cell or tissue samples. Helps study mTOR signaling pathway activity, which plays a key role in cell growth, proliferation, survival, and metabolism. Widely applied in cancer research, metabolic disease studies, and drug development to assess the effects of therapeutic agents on mTOR activation. Enables researchers to monitor changes in phosphorylation status in response to stimuli such as growth factors, nutrients, or inhibitors like rapamycin. Provides a reliable and sensitive method for high-throughput analysis in preclinical studies.