Used in immunological research to quantify perforin levels in biological samples, aiding in the study of cytotoxic T-cell and natural killer (NK) cell activity. Helps assess immune responses in conditions such as viral infections, autoimmune diseases, and cancer. Commonly applied in clinical research and drug development to monitor changes in perforin expression during therapy or disease progression. Enables high-throughput analysis of perforin release in cell culture supernatants and serum samples.