Used in immunological research to detect and quantify levels of autoantibodies against histidyl tRNA synthetase (Jo-1) in serum samples. Commonly applied in studies of autoimmune diseases, particularly idiopathic inflammatory myopathies such as polymyositis and dermatomyositis. Helps in diagnosing Jo-1 positive patients and monitoring disease activity. Also used in clinical laboratories and research settings to investigate the role of anti-synthetase antibodies in interstitial lung disease and other connective tissue disorders. Provides a reliable, high-throughput method for antibody screening in large patient cohorts.