Used in biomedical research to quantify levels of Ryanodine Receptor 1 (RYR1) in rat tissue samples, particularly in studies related to skeletal muscle function and calcium signaling. Enables researchers to assess RYR1 expression changes in experimental models of muscle disorders such as malignant hyperthermia, muscular dystrophy, and exercise-induced fatigue. Commonly applied in pharmacological studies to evaluate drug effects on intracellular calcium release mechanisms. The kit supports high-throughput analysis in preclinical research, aiding in the development of therapeutics targeting calcium channel dysfunction.