Used to detect and quantify rat immunoglobulin G2a (IgG2a) in biological samples such as serum, plasma, or cell culture supernatants. Commonly applied in immunological research to assess immune responses, particularly in studies involving rat models of infection, vaccination, or autoimmune diseases. The kit enables researchers to measure antigen-specific IgG2a levels, which is often associated with a Th1-type immune response. It is also used in pharmacology and toxicology studies to evaluate the immunogenicity of drugs or biologics in rats. The assay is typically performed in 96-well plates, allowing high-throughput analysis with high specificity and sensitivity.