Used to measure levels of vascular endothelial growth factor (VEGF) in biological samples such as serum, plasma, or tissue homogenates from rats. This assay is widely applied in research related to angiogenesis, inflammation, and tumor development. It helps in evaluating the effects of experimental treatments on VEGF expression in disease models including cancer, diabetic retinopathy, and ischemic injuries. The kit provides a sensitive and specific way to quantify VEGF protein, supporting studies in pharmacology, toxicology, and molecular biology.