Used to measure rat epidermal growth factor (EGF) levels in biological samples such as serum, plasma, and cell culture supernatants. Commonly applied in research settings to study cell proliferation, tissue repair, and inflammation in rat models. Helps in evaluating the role of EGF in wound healing, cancer development, and kidney or liver injury studies. Provides a sensitive and specific quantification method for monitoring changes in EGF expression under experimental conditions.