Used to detect and quantify mouse α-smooth muscle actin (α-SMA) protein levels in biological samples such as cell lysates, tissue homogenates, and serum. Widely applied in research on fibrosis, where α-SMA serves as a marker for activated myofibroblasts. Enables monitoring of disease progression in models of liver, lung, kidney, and cardiac fibrosis. Also utilized in cancer research to study tumor stroma and epithelial-mesenchymal transition (EMT). Provides a reliable, sensitive, and specific method for high-throughput screening in preclinical studies and drug development targeting fibrotic diseases or tissue remodeling.