Used in immunology and cancer research to quantify sphingosine 1-phosphate levels in biological samples such as serum, plasma, and cell culture supernatants. Enables study of S1P's role in immune cell trafficking, inflammation, angiogenesis, and autoimmune diseases. Commonly applied in preclinical studies involving mouse models to evaluate therapeutic interventions targeting the S1P signaling pathway. Supports high-throughput screening and biomarker discovery in neuroinflammatory and cardiovascular research.