Used in biomedical research to quantify Substance P levels in biological samples such as tissue homogenates, cell cultures, and cerebrospinal fluid. Widely applied in neuroscience studies to investigate pain transmission, neuroinflammation, and nervous system disorders. Also utilized in immunology and pharmacology research to evaluate the effects of drugs or treatments on neuropeptide release. The kit enables high-throughput, sensitive, and specific detection, making it suitable for both academic and preclinical research settings.