Used in biomedical research to quantify SH2B1 protein levels in cell lysates, tissue extracts, and biological fluids such as serum or plasma. Enables investigation of SH2B1's role in signaling pathways related to metabolism, insulin sensitivity, and leptin signaling. Commonly applied in studies of obesity, diabetes, and metabolic syndrome. Supports high-throughput screening in drug development and validation of SH2B1 expression changes under experimental conditions. Provides a reliable, sensitive, and specific measurement method for both basic research and preclinical studies.