Used in neuroscience and clinical research to quantify S100B protein levels in biological samples such as serum, plasma, and cerebrospinal fluid. Helps assess blood-brain barrier integrity and glial cell activity. Commonly applied in studies related to traumatic brain injury, stroke, neurodegenerative diseases, and neonatal encephalopathy. Also utilized in monitoring disease progression and evaluating therapeutic interventions in brain disorders. Provides a sensitive and specific method for detecting low concentrations of S100B, supporting both academic research and drug development.