Used in immunological research to quantify SOCS-1 protein levels in biological samples such as cell lysates, serum, or tissue extracts. Helps study the regulation of cytokine signaling pathways, particularly in inflammation, autoimmune diseases, and immune responses. Commonly applied in studies related to JAK/STAT pathway modulation, where SOCS-1 acts as a negative regulator. Enables researchers to assess immune cell activity and signaling feedback mechanisms in experimental models of disease. Widely used in preclinical research for developing therapies targeting immune dysregulation.