Used in biomedical research to quantify phosphorylated SMAD3 protein levels in cell or tissue samples. Enables researchers to study TGF-β signaling pathway activation, which plays a key role in cellular processes such as proliferation, differentiation, apoptosis, and fibrosis. Commonly applied in studies related to cancer, immune disorders, and organ fibrosis. Provides a reliable, sensitive, and high-throughput method for monitoring SMAD3 activation in response to stimuli or drug treatments. Suitable for use in preclinical research and mechanistic studies in molecular and cell biology.