Used in biomedical research to detect and quantify α-smooth muscle actin in tissue samples and cell cultures. Helps in studying activation of fibroblasts and myofibroblasts, which play key roles in tissue fibrosis, wound healing, and cancer progression. Commonly applied in research on liver cirrhosis, pulmonary fibrosis, kidney disease, and cardiovascular conditions. Enables researchers to assess the extent of fibrotic changes and evaluate potential antifibrotic therapies. Suitable for use with serum, plasma, and tissue homogenates in experimental models.