Used in biomedical research to quantify TIMP-1 protein levels in biological samples such as serum, plasma, and cell culture supernatants. It plays a key role in studying tissue remodeling, inflammation, and diseases involving extracellular matrix degradation, including cancer, liver fibrosis, and cardiovascular conditions. The kit enables researchers to assess TIMP-1's role as a natural inhibitor of matrix metalloproteinases (MMPs), helping to understand disease progression and evaluate potential therapeutic interventions. Widely applied in preclinical studies and biomarker validation due to its sensitivity and specificity.