Used to quantify TIMP-3 protein levels in biological samples such as serum, plasma, cell lysates, and tissue homogenates. Widely applied in research on extracellular matrix remodeling, inflammation, and tissue degradation. Helps in studying diseases like arthritis, cardiovascular disorders, fibrosis, and cancer where TIMP-3 plays a regulatory role in matrix metalloproteinase activity. Provides a reliable, sensitive, and specific measurement for monitoring disease progression and evaluating therapeutic interventions in experimental models.