Used in biomedical research to quantify levels of TIMP-2 in biological samples such as serum, plasma, and cell culture supernatants. This kit enables researchers to study the role of TIMP-2 in tissue remodeling, cancer progression, fibrosis, and inflammatory diseases. By measuring TIMP-2 concentration, scientists can assess its interaction with matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, which are involved in extracellular matrix degradation. The assay supports investigations in oncology, cardiovascular diseases, and autoimmune disorders where imbalance between TIMPs and MMPs contributes to disease mechanisms. It is also applied in drug development to evaluate therapeutic agents targeting protease activity.